NANOSECOND PULSED ELECTRIC FIELD MODULATES IMMUNOPHENOTYPE OF LYMPHOCYTES

Introduction: Nanosecond pulsed electric fields (nsPEFs) have been shown to anticancer effects, but little is known about the mechanisms modulating immune landscape. According applied parameters (voltage, pulse duration, delivery frequency, and a number of pulses), it possible achieve specific effect immunophenotype cancer cells. This study aims identify changes in expression profile antigens defining lymphocyte maturity function. Materials Methods: We treated Jurkat cell line with PEF. tested different electroporation (2.5, 5, 7.5, 10, 12.5 kV/cm, 100, 200 100 ns pulses, 25, 250, 500 1, 10 Hz). For detection permeabilization, Yo-Pro-1 flow cytometry were employed. Cell viability was analyzed after 24h by MTT Assay. ATP levels culture measured using Cell-Titer-Glo® Assay 24 hours. The markers determined cytometry. Results: obtained results confirmed that permeabilizes membrane modulates Moreover, we observed release increases when higher are applied. Application 10kHz-100Hz 25 pulses nsPEF induces an increase CD154 costimulatory molecule, which regulates response priming T cells activating Simultaneously, early activation marker CD69, indicating ability activate lymphocytes. application 10kHz parameter upregulated involved recruitment inflammatory (CD183), controlling homeostasis peripheral lymphocytes (CD127) regulating platelet adhesion (CD61). Importantly, thus noted there no alteration CD95, can mediate induction apoptosis On contrary, significant CD7 subexpression 10Hz In general, not undergone death lower field modified their immunophenotype. Based on our studies, propose mechanism cells: (1) permeabilize membrane, (2) lymphocyte-activation antigens, (3) downregulate CD7. Conclusions: Concluding, state be promising approach immunotherapy via enhancing presentation antigens. Also, proved treatment could modulate while avoiding death. Encore Abstract - previously submitted regional or national meetings (up < 1’000 attendees) research funded by: founded Polish National Centre Science DAINA 2 (2020/38/L/NZ7/00342; PI: J. Kulbacka), Research Council Lithuania grant (Nr. S-LL-21-4, V. Novickij). Keywords: Diagnostic Prognostic Biomarkers, Basic Translational Other No conflicts interests pertinent abstract.